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-Development of methods to enhance the rate of toxin depuration ( detoxification), especially in shellfish species of high economic value and prolonged retention e.g., King Scallops -Understanding the reaction products and metabolic transformations of toxins in shellfish tissues. -Determine the relationship between algal population dynamics ( including free cell and encysted stages ) to seasonal and spatial patterns of toxicity in shellfish populations. -Assess the effects of harmful algae on the various stages in the life history of shellfish ( Larvae, Spat, Adults ). -Investigate sampling frequencies and protocols ( live shellfish sampling ).
-Development of methods to enhance the rate of toxin depuration ( detoxification), especially in shellfish species of high economic value and prolonged retention e.g., King Scallops -Understanding the reaction products and metabolic transformations of toxins in shellfish tissues. -Determine the relationship between algal population dynamics ( including free cell and encysted stages ) to seasonal and spatial patterns of toxicity in shellfish populations. -Assess the effects of harmful algae on the various stages in the life history of shellfish ( Larvae, Spat, Adults ). -Investigate sampling frequencies and protocols ( live shellfish sampling ).
1. To descirbe and compare the phylogenetic diversity and distribution of the total bacterial flora associated with G catenatum cysts and vegetative cells. 2. To culture and identify bacteria from G catenatum, and identify/characterise any bacteria capable of autonomous PST production in G. catenatum 3. To examine the effect of cyst surface sterilisation and re-introduction of bacteria on PST production in G catenatum 4. Survey bacteria for quorum sensing capability (cell signaling) and detect in situ quorum sesing in xenic G. catenatum cultures, relating to toxicity development. 5. Develop molecular markers of cross species quorum sensing, facilitating analysis of quorum sensing in uncultivated bacteria.